A laboratory pilot study on voids in flowable bulk-fill composite restorations in bovine Class-II and endodontic access cavities after sonic vibration.

This pilot study investigated whether sonic-powered application of a bulk-fill resin-based composite (RBC) in Class-II or endodontic access cavities reduces void formation. The crowns and roots of 60 bovine teeth with Class-II cavities (C) and endodontic access cavities (E) respectively, were assigned to ten groups (C1-C5, E1-E5). Cavities were filled with RBC (SDR flow + , one increment) using different application techniques: no adaptation (C1 + E1), spreading of RBC on the cavity surfaces with a dental explorer tip (C2 + E2), low (C3 + E3) or high frequency (C4 + E4) direct activation by inserting a sonic-powered tip into RBC and high frequency indirect activation with an ultrasonic insertion tip (C5 + E5). The restorations were light-cured and investigated for voids using microtomography. The number of voids and percentage of voids related to the volume were statistically analysed (α < 0.05). While most voids in Class-II restorations were observed in C4 (p ≤ 0.0031), no significant differences were found between the other groups (p > 0.05). The percentage of voids showed no differences in E1-E5 (p > 0.05). C4 showed a significantly higher percentage of voids compared to C2 (p < 0.001). There is no benefit in applying sonic vibration when filling Class-II or endodontic access cavities.

Effect of brushing force on the abrasive dentin wear using slurries with different abrasivity values.

OBJECTIVE: The aim of this study was to investigate the resulting abrasive dentin wear using abrasive slurries with different RDA values and applying increasing brushing forces. METHODS: Forty-five bovine incisors were randomly allocated in three groups (A, B, C, n = 15). One hundred and eighty dentin samples were prepared from these incisors and allocated to twelve groups (A1-A4, B1-B4, C1-C4; n = 15). The groups were subjected to a brushing cycle (120 strokes/min, 25 min) as follows: groups A1 to A4 with an abrasive slurry (RDA = 71) applying increasing brushing forces (1, 2, 3 and 4 N). Groups B1 to B4 were brushed using an abrasive slurry (RDA = 85) and C1 to C4 (RDA = 133) applying the same above-mentioned brushing forces. Abrasive dentin wear was recorded using a stylus profilometer and compared amongst the groups using robust models. Pairwise comparisons in each model were tested and corrected after Tukey's method (α = 0.05). RESULTS: Applying 1-N brushing force resulted in the same amount of abrasive dentin wear in all groups regardless of the abrasivity of the used slurry. Increasing the brushing force to 2 N resulted in statistically significantly higher abrasive wear in all groups. This increase in abrasive wear was much higher when the slurry with high abrasivity was used (RDA = 133) compared with the lower abrasive slurry (RDA = 71). CONCLUSION: The abrasivity of the used slurry does not add to the resulting abrasive wear when the brushing force is kept at 1 N. It seems better to advise and help the patients (showing signs of non-carious cervical lesions) calibrating their brushing force to 1 N, than only to advise them to use toothpastes with lower abrasivities.

Effect of tapered-end and round-end bristles on the abrasive dentine wear applying increasing brushing forces.

OBJECTIVE: To investigate the effect of toothbrush bristles end configuration on the abrasive dentine wear at different brushing forces. MATERIAL AND METHODS: One hundred and sixty bovine dentine samples were randomized into eight groups (n = 20). Groups (1 to 4) were brushed with tapered-end bristles at 1-, 2-, 3-, and 4-N brushing forces, respectively. Groups (5 to 8) were brushed with round-end bristles with the same brushing forces. The brushing sequence was carried out using an abrasive slurry (RDA = 121) for 25 min. Profiles were recorded using a contact profilometer. Mean and standard deviation were calculated for the abrasive dentine wear in each group. Two-way ANOVA was used to locate any significance. The significance values were corrected after Tukey (α = 0.05). RESULTS: Brushing with tapered-end bristles resulted in statistically significantly less abrasive dentine wear than round-end bristles regardless of the applied brushing force (1 N: 4.4 ± 1.5 vs. 7.7 ± 2.0 µm; 2 N: 7.7 ± 2.1 vs. 12.2 ± 2.7 µm; 3 N: 11.3 ± 2.6 vs. 19.4 ± 3.7 µm; 4 N: 11.2 ± 2.1 vs. 25.3 ± 4.8 µm). The interaction between bristle configuration and brushing force was statistically significantly (p < 0.001). CONCLUSION: Tapered-end toothbrushes might be a safer choice for patients, especially when showing signs of non-carious cervical lesion. However, other toothbrush properties should also be considered.

Effect of the toothbrush tuft arrangement and bristle stiffness on the abrasive dentin wear.

The geometrical properties of toothbrushes play a role in developing abrasive tooth wear and non-carious cervical lesions. This study investigated the interplay between the toothbrush tuft arrangement (crossed vs. parallel) and bristle stiffness (soft vs. medium) on the abrasive dentin wear using three slurries with different levels of abrasivity (RDA: 67, 121 and 174). Twelve groups of bovine dentin samples (n = 20) were brushed with a combination of the aforementioned variables. Abrasive dentin wear was recorded with a profilometer and the resulting abrasive wear of each group was calculated and compared with each other using two-way ANOVA and pairwise tests. Toothbrushes with parallel tuft arrangement caused statistically significantly higher dentin wear compared to crossed tuft arrangement, regardless of the abrasivity level of the used slurry and the bristle stiffness. Soft crossed tuft toothbrushes caused statistically significantly higher abrasive dentin wear than medium crossed tuft toothbrushes, while soft and medium parallel tuft toothbrushes caused the same amounts of dentin wear, regardless of the RDA value of the used slurry. These results could be helpful for dentists and dental hygienists when advising patients. Crossed tuft toothbrushes could be a less-abrasive choice in comparison to parallel tuft toothbrushes.

OralDisk: A Chair-Side Compatible Molecular Platform Using Whole Saliva for Monitoring Oral Health at the Dental Practice.

Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients' general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) and three caries-associated (oral Lactobacilli, Streptococcus mutans, Streptococcus sobrinus). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk's potential and suitability for inclusion in larger prospective implementation studies in dental care settings.

Enamel Softening Can Be Reduced by Rinsing with a Fluoride Mouthwash Before Dental Erosion but Not with a Calcium Solution.

PURPOSE: This in-situ-study investigated if rinsing the oral cavity with a calcium containing solution or a fluoride containing mouthwash immediately before an erosive attack leads to reduced enamel softening. MATERIALS AND METHODS: Bovine enamel samples (n = 240) with measured baseline surface microhardness (KHN) were assigned to five series (S1-5). Twelve participants carried out each series as follows: Four enamel samples of the associated test series were placed in an intraoral appliance and carried in each participants' mouth. After 30 min, the participants either rinsed the oral cavity for 60 s with 30 ml of a solution prepared from a 1,000 mg calcium effervescent tablet dissolved in 100 ml water (S2), an 800 mg calcium containing mineral supplement powder (5 g) dissolved in 200 ml water (S3), a fluoride (500 ppm) mouthwash (S4), a fluoride (500 ppm) and stannous chloride (800 ppm) containing mouthwash (S5), or did not rinse with any test solution before the erosive attack (S1, negative control). The participants subsequently rinsed the oral cavity with 100 ml of a soft drink (Sprite Zero) for 60 s to simulate the erosive attack and immediately afterwards with water to stop the erosive process. As final step, surface microhardness was measured a second time and hardness loss (∆KHN) calculated. Differences of ∆KHN between the series were investigated by fitting a mixed effect model to the data set. RESULTS: The highest loss of microhardness and thus softening of enamel (mean of ∆KHN; lower/upper confidence level) was observed in the negative control (S1: 60.2; 67.6/52.8). While no statistically significant difference (P > 0.05) compared to S1 could be found in S2 (50.0; 57.4/42.5) and S3 (54.6; 62.1/47.2), statistically significantly less softening of enamel (P < 0.001) was discovered in S5 (33.8; 41.2/26.4) and S4 (41.8.2; 49.3/34.4). S5 showed the overall lowest values for ∆KHN and thus best protection from enamel softening. CONCLUSION: Rinsing with a fluoride mouthwash or a fluoride and stannous chloride containing mouthwash immediately before an erosive attack reduces the softening of enamel. None of the investigated calcium-containing solutions was able to reduce erosion induced softening of enamel.

Effect of Caries Infiltrant on Margin Integrity of Composite Fillings Placed Adjacent to Demineralised Primary Enamel.

PURPOSE: To investigate the influence of pretreating demineralised enamel with an infiltrant on the margin integrity of Class V like composite restorations on primary teeth bonded with different adhesives. MATERIALS AND METHODS: Forty specimens from primary molars were demineralised and circular class-V-like cavities were prepared. The cavities were treated with a universal adhesive (Scotchbond Universal Adhesive, 3M Oral Care), applied either in self-etch (SE) or etch-and-rinse mode (ER) mode. In groups SE-I and ER-I, the demineralised margins were pretreated with a caries infiltrant (Icon, DMG) prior to adhesive application. The cavities were restored with a nanofilled composite material and thermocycled. Marginal integrity was evaluated using SEM, and the percentage of continuous margin was statistically analysed. RESULTS: Specimens treated with the caries infiltrant followed by the adhesive showed similar marginal continuity as the adhesive alone. CONCLUSIONS: Pretreatment of demineralised primary enamel with a caries infiltrant before applying a universal adhesive does not influence the marginal integrity of composite fillings.

Erosive tooth wear among athletes.

The following article deals with various considerations concerning the frequently observed occurrence of dental erosion in athletes. It summarizes the basics of erosive tooth wear development as well as special etiologic factors for groups of athletes with increased prevalence of dental erosion and concludes with preventive advice. (Originally published (in German) in Quintessenz Zahnmed 2021;72:1008-1017).

Green Tea Extract Reduces the Erosive Dentine Wear Caused by Energy Drinks In Vitro.

PURPOSE: To investigate the effect of energy drinks supplementation with green tea extract on the erosive dentine wear. MATERIALS AND METHODS: Six groups of bovine dentine samples (n = 15) were subjected to four cycles erosive attacks (10 min, 25 °C) and remineralisation (artificial saliva, 60 min, 37°C) using the following formulas: tap water; green tea extract; Red Bull; Red Bull supplemented with green tea extract; Red Bull Light; Red Bull Light supplemented with green tea extract. The erosive dentine wear - ie, the irreversible dentine loss - was measured using a stylus profilometer (µm, accuracy = 40 nm). RESULTS: Median and interquartile range (IQR) of erosive dentine wear for the tested energy drinks before and after the supplementation with green tea extract were calculated as follows: Red Bull (before: 3.3 µm (1.0)); after: 1.2 µm (0.6)); Red Bull Light (before: 3.3 µm (0.9)); after: 2.0 µm (0.4)). The difference between the groups before and after the supplementation was statistically significant (P ˂0.05). The erosive dentine wear for the tap water group was calculated at 0.4 µm (0.6) and for the green tea extract group at -1.0 µm (1.3). CONCLUSIONS: Supplementation of energy drinks with green tea extract could reduce the erosive dentine wear caused by energy drink in vitro.

Effect of toothbrush bristle stiffness and toothbrushing force on the abrasive dentine wear.

OBJECTIVE: To investigate the influence of toothbrush bristle stiffness and applied brushing force on the resulting abrasive dentine wear in vitro. METHODS: One hundred sixty bovine dentine samples were randomly allocated in eight groups (n = 20). Groups one to four were brushed with a soft-bristle toothbrush with soft bristles applying 1, 2, 3 and 4 N brushing force, respectively. Groups five to eight were brushed with a medium-bristle toothbrush applying the same aforementioned brushing forces (120 strokes/min, abrasive slurry (RDA = 121), 25 min). Profiles were recorded before and after the brushing sequence, and the median and interquartile range of abrasive dentine wear were calculated and compared using two-way ANOVA and pairwise tests corrected after Tukey (α = 0.05). RESULTS: At 1, 2 and 3 N brushing force, the tested toothbrushes caused no significantly different abrasive dentine wear. At 4 N brushing force, the medium-bristle toothbrush caused statistically significantly higher abrasive force than the soft-bristle toothbrush. Using the medium-bristle toothbrush, abrasive dentine wear statistically significantly increased with increasing brushing force from 1 to 3 N. However, increasing the brushing force to 4 N did not result in statistically significantly higher wear. Using the soft-bristle toothbrush, abrasive dentine wear statistically significantly increased with increasing brushing force from 1 to 2 N. However, increasing the brushing force to 3 or 4 N did not result in statistically significantly higher wear. CONCLUSION: The soft-bristle toothbrush tends to cause less abrasive wear with increasing brushing force than the medium-bristle toothbrush.

Effect of dentin pretreatment on the resulting abrasive dentin wear.

BACKGROUND: This study aims to investigate the influence of different dentin pretreatment procedures on the resulting abrasive dentin wear. METHODS: Two groups (A, B) of 60 dentin samples each were prepared. Group A was brushed with an abrasive slurry (RDA = 85) and group B with a different abrasive slurry (RDA = 174). Four subgroups in each group (n = 15) were created (A1-A4) and (B1-B4). The subgroups were pretreated as follows: A1 + B1 with 1200-grit grinding paper, A2 + B2 with 1200- and 2000-grit papers, A3 + B3 with 1200-, 2000-, and 4000-grit papers, A4 + B4 with 1200-, 2000-, 4000-grit papers and with 1000 brushing strokes with a slurry of Elmex toothpaste. All samples were brushed for 25 min at 120 strokes/min. Abrasive dentin wear was measured for each sample profilometrically and the subgroups were compared with each other within the same group. Repeated measures one-way ANOVA was conducted to compare the subgroups and pairwise contrasts were estimated for multiple testing according to Tukey (α = 0.05). RESULTS: The resulting abrasive dentin wear in group A ranged between 15.3 ± 3.4 µm and 17.3 ± 5.5 µm and between 20.3 ± 6.8 µm and 22.5 ± 2.6 µm in group B. No statistically significant difference was noticed between any subgroups within the same group (p ˃ 0.05). CONCLUSIONS: Different dentin pretreatment procedures do not affect the resulting abrasive dentin wear independent of the RDA value of the employed abrasive slurry.

Effect of a sonic toothbrush on the abrasive dentine wear using toothpastes with different abrasivity values.

OBJECTIVE: This in vitro study aimed to investigate the effect of a sonic toothbrush on the resulting abrasive dentine wear using toothpastes with different abrasivities compared to the use of a manual toothbrush. METHODS: Ninety-six bovine dentine samples were divided into six groups and subjected to a brushing sequence (sonic: 20 min, 0.9 N, 10 strokes/min; manual: 20 min, 1.6 N, 60 strokes/min) as follows: group 1: Elmex Sensitive Plus (RDA = 28) + manual toothbrush; group 2: Elmex Sensitive + sonic toothbrush; group 3: Elmex Kariesschutz (RDA = 65) + manual toothbrush; group 4: Elmex Kariesschutz +sonic toothbrush; group 5: Colgate Total Original (RDA = 121) + manual toothbrush; and group 6: Colgate Total Original +sonic toothbrush. The abrasive dentine wear was measured profilometrically. RESULTS: The median (IQR) abrasive dentine wear was as follows: Elmex Sensitive Plus (manual toothbrush: 2.7 µm (0.8), sonic toothbrush: 3.1 µm (1.9)); Elmex Kariesschutz (manual toothbrush: 4.9 µm (1.4), sonic toothbrush: 6.4 µm (2.1)); and Colgate Total Original (manual toothbrush: 5.2 µm (1.1), sonic toothbrush: 9.0 µm (3.1)). Differences in dentine wear between sonic and manual toothbrushes were statistically significant only in the groups brushed with Elmex Kariesschutz and Colgate Total Original (p ≤ 0.05). CONCLUSION: Brushing with a sonic toothbrush could result in a higher abrasive dentine wear compared to manual toothbrush when combined with toothpastes with high abrasivity values.

Potential of different fluoride gels to prevent erosive tooth wear caused by gastroesophageal reflux.

BACKGROUND: This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. METHODS: Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1-5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2-5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. RESULTS: The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). CONCLUSIONS: After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.

Salivary Biomarkers for Dental Caries Detection and Personalized Monitoring.

This study investigated the potential of salivary bacterial and protein markers for evaluating the disease status in healthy individuals or patients with gingivitis or caries. Saliva samples from caries- and gingivitis-free individuals (n = 18), patients with gingivitis (n = 17), or patients with deep caries lesions (n = 38) were collected and analyzed for 44 candidate biomarkers (cytokines, chemokines, growth factors, matrix metalloproteinases, a metallopeptidase inhibitor, proteolytic enzymes, and selected oral bacteria). The resulting data were subjected to principal component analysis and used as a training set for random forest (RF) modeling. This computational analysis revealed four biomarkers (IL-4, IL-13, IL-2-RA, and eotaxin/CCL11) to be of high importance for the correct depiction of caries in 37 of 38 patients. The RF model was then used to classify 10 subjects (five caries-/gingivitis-free and five with caries), who were followed over a period of six months. The results were compared to the clinical assessments of dental specialists, revealing a high correlation between the RF prediction and the clinical classification. Due to the superior sensitivity of the RF model, there was a divergence in the prediction of two caries and four caries-/gingivitis-free subjects. These findings suggest IL-4, IL-13, IL-2-RA, and eotaxin/CCL11 as potential salivary biomarkers for identifying noninvasive caries. Furthermore, we suggest a potential association between JAK/STAT signaling and dental caries onset and progression.

Toothpastes with Enzymes Support Gum Health and Reduce Plaque Formation.

Enzymes in toothpastes can support host immune responses, and thus maintain oral health. This study aimed to investigate gingival health and the plaque-reducing effects of enzyme-containing toothpastes. A laboratory study tested the antimicrobial potential of different enzyme-containing toothpaste formulations. Two promising formulations (enzyme-containing toothpastes with glucose oxidase and D-glucose with (C+) and without Citrox (C-) Citrox) were investigated in a clinical crossover trial (two slurries: sodium lauryl sulfate-containing (SLS), a toothpaste without SLS (reference), and water). Subjects (n = 20) abstained from toothbrushing for four days and rinsed with a toothpaste slurry. Bleeding on probing (BOP) and plaque indices (PI) were measured. A mixed linear model was used to statistically compare the slurries with respect to BOP and PI change. The in vitro bacterial growth-inhibiting evaluation showed the best results for SLS, followed by C+ and C-. The change in BOP and PI exhibited statistically significant differences to water rinsing (BOP; PI changes in % points (difference of the baseline and post-rinse values: water = 8.8%; 90.0%; C+ = -1.4%; 80.4%; SLS = 1.5%; 72.1%; reference = 0.8%; 77.5%; C- = -1.8%; 75.1%). All slurries exhibited anti-gingivitis and anti-plaque effects, resulting in a prophylactic benefit for limited-access regions during brushing.

[Assessment of dentists' opinions on the cause of failure of adhesive composite restorations]. / Einschätzung von Zahnärzten zu vermuteten Gründen für das Scheitern von Kompositfüllungen mittels Adhäsivtechnik.

Aim of this study was to get an overview of what dentists estimate to be the reason for failure of adhesive composite restorations and to gain knowledge about potential future foci for targeted further education. Therefore, a survey about the fabrication of composite restorations was conducted in the course of seven dental conferences with the main topic tooth-prevention. A total of 577 dentists participated in the survey. The most often called estimated factor for failure of composite restorations was the "dentist" (70.7%), followed by "indication" (32.2%), "material" (8.5%) and "patient" (8.1%). Participants estimated insufficient drying and fluid management (67.9%), incorrect application of the adhesive system (41.8%), insufficient lightpolymerisation (31.2%) and errors in incremental technique (27.6%) to be the main mistakes. The participating dentists claimed to attach great importance to careful drying and fluid management (60.8%), thorough adhesive application (37.3%), adequate incremental technique (32.6%) and sufficient lightpolymerisation (28.8%) while placing their own restorations. According to the survey participants, the treating dentist has the greatest influence on the quality and longevity of composite restorations. Drying and fluid management was the most often called estimated reason for failure but at the same time the step which is given the greatest caution.

Effects of Additional Use of Bioactive Glasses or a Hydroxyapatite Toothpaste on Remineralization of Artificial Lesions in vitro.

OBJECTIVES: This in vitro study aimed to evaluate and compare the effect of two different bioactive glasses, a hydroxyapatite-containing, fluoride-free toothpaste (HTP) and a fluoride toothpaste (FTP) on the remineralization behavior of initial caries lesions. MATERIALS AND METHODS: A total of 100 bovine enamel samples were randomly allocated to five groups of 20 samples each: NC = negative control group (artificial saliva); HTP = HTP group (Karex); FTP = FTP group (Elmex caries protection, 1,400 ppm); FTP + BGnano = FTP followed by Actimins bioactive glass; FTP + BGamorph = FTP followed by Schott bioactive glass. Radiographic documentation (advanced transversal microradiography; aTMR) was applied before and after all samples were exposed to a demineralizing gel for 10 days. Over a period of 28 days, samples were covered twice a day (every 12 h) with a toothpaste slurry of the respective test group or with artificial saliva in NC for 60 s and brushed with 15 brushing strokes. Samples in FTP + BGnano and FTP + BGamorph were additionally treated with the respective bioactive glass slurry for 30 s after brushing with the FTP. In the meantime, all samples were stored in artificial saliva. After 28 days, the structure of all samples was assessed again using aTMR and compared to the values measured after demineralization. The statistical evaluation of the integrated mineral loss was performed using Kruskal-Wallis test followed by a post hoc Conover test. RESULTS: The FTP revealed the significantly highest increase of mineral content while the HTP showed the significantly lowest remineralization. Compared to artificial saliva, the use of the HTP or the combined application of FTP followed by bioactive glasses (FTP + BGnano and FTP + BGamorph) showed no significant remineralization. CONCLUSION: Under remineralizing in vitro conditions, brushing with 1,400 ppm FTP induced significantly more remineralization compared to storage in artificial saliva. The additional administration of both bioactive glasses as well as the substitutional brushing with an HTP resulted in significantly less remineralization compared to brushing with 1,400 ppm FTP.

Microbial Analysis of Saliva to Identify Oral Diseases Using a Point-of-Care Compatible qPCR Assay.

Oral health is maintained by a healthy microbiome, which can be monitored by state-of-the art diagnostics. Therefore, this study evaluated the presence and quantity of ten oral disease-associated taxa (P. gingivalis, T. forsythia, T. denticola, F. nucleatum, C. rectus, P. intermedia, A. actinomycetemcomitans, S. mutans, S. sobrinus, oral associated Lactobacilli) in saliva and their clinical status association in 214 individuals. Upon clinical examination, study subjects were grouped into healthy, caries and periodontitis and their saliva was collected. A highly specific point-of-care compatible dual color qPCR assay was developed and used to study the above-mentioned bacteria of interest in the collected saliva. Assay performance was compared to a commercially available microbial reference test. Eight out of ten taxa that were investigated during this study were strong discriminators between the periodontitis and healthy groups: C. rectus, T. forsythia, P. gingivalis, S. mutans, F. nucleatum, T. denticola, P. intermedia and oral Lactobacilli (p < 0.05). Significant differentiation between the periodontitis and caries group microbiome was only shown for S. mutans (p < 0.05). A clear distinction between oral health and disease was enabled by the analysis of quantitative qPCR data of target taxa levels in saliva.

Erosive/Abrasive Enamel Wear While Using a Combination of Anti-Erosive Toothbrush/-Paste.

PURPOSE: To investigate dental enamel wear caused by erosion and abrasion while using a combination of anti-erosive toothbrush/-paste. MATERIALS AND METHODS: A total of 60 enamel specimens from bovine incisors were randomly assigned into five groups of 12 specimens each (G1-5, n = 12): (G1) control group (no treatment), (G2) standard medium toothbrush Paro M43 and standard toothpaste Elmex Caries Protection, (G3) standard medium toothbrush Paro M43 and anti-erosive toothpaste Elmex Protection Erosion, (G4) anti-erosive toothbrush Elmex Erosion Soft and standard toothpaste Elmex Caries Protection, (G5) anti-erosive toothbrush Elmex Erosion Soft and anti-erosive toothpaste Elmex Protection Erosion. Initially, surface baseline profiles were recorded using profilometry. In a total of 60 cycles, all specimens were exposed to hydrochloric acid (pH = 3) for 1 min, rinsed with tap water to stop the erosive attack and brushed according to the specific protocol of each group (15 brushing strokes per run). Enamel loss was determined by comparing the surface profiles before and after 60 cycles and the results were statistically analysed using analysis of variance (ANOVA). RESULTS: The significantly highest loss of enamel was observed in the control group G1(1.4 ± 0.20 µm) (p < 0.001). G2 turned out to be the most abrasive toothbrush/-paste combination (1.12 ± 0.15 µm), G3 the least invasive (0.40 ± 0.04 µm) (p < 0.001, respectively). CONCLUSION: All combinations of the investigated toothbrushes/-pastes reduce erosive/abrasive enamel wear. However, the highest reduction was observed for the combination of anti-erosive toothpaste and standard toothbrush (G3).